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amplican

Automated analysis of CRISPR experiments

Bioconductor version: 3.24 · Package version: 1.35.5

`amplican` performs alignment of the amplicon reads, normalizes gathered data, calculates multiple statistics (e.g. cut rates, frameshifts) and presents results in form of aggregated reports. Data and statistics can be broken down by experiments, barcodes, user defined groups, guides and amplicons allowing for quick identification of potential problems.

Installation

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("amplican")

Details

MaintainerEivind Valen <eivind.valen@gmail.com>
AuthorKornel Labun [aut], Eivind Valen [cph, cre]
LicenseGPL-3
URLhttps://github.com/valenlab/amplican
Bug Reportshttps://github.com/valenlab/amplican/issues
Downloads rank683
Source branchdevel
biocViewsAlignment, CRISPR, ImmunoOncology, Software, Technology, qPCR

Documentation

Download

Follow the installation instructions to use this package in your R session.

Source packageamplican_1.35.5.tar.gz
Windows binary (x86_64)amplican_1.35.5.zip
macOS binary (arm64)amplican_1.35.5.tgz
macOS binary (x86_64)amplican_1.35.5.tgz
Dependencies

Depends: R (>= 3.5.0), methods, BiocGenerics (>= 0.22.0), Biostrings (>= 2.44.2), pwalign

Imports: Rcpp, utils (>= 3.4.1), S4Vectors (>= 0.14.3), ShortRead (>= 1.34.0), IRanges (>= 2.10.2), GenomicRanges (>= 1.61.1), Seqinfo, BiocParallel (>= 1.10.1), gtable (>= 0.2.0), gridExtra (>= 2.2.1), ggplot2 (>= 3.3.4), ggthemes (>= 3.4.0), stringr (>= 1.2.0), stats (>= 3.4.1), matrixStats (>= 0.52.2), Matrix (>= 1.2-10), data.table (>= 1.10.4-3), rmarkdown (>= 1.6), knitr (>= 1.16), cluster (>= 2.1.4), rlang (>= 1.2.0)

LinkingTo: Rcpp

Suggests: testthat, BiocStyle, GenomicAlignments