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GExPipe

GExPipe: Gene Expression Pipeline Shiny Application

Bioconductor version: 3.24 · Package version: 0.99.51

Shiny application (GExPipe) for high-throughput genomic analysis of bulk RNA-seq and microarray data (e.g. from GEO). Integrates with Bioconductor (GEOquery, Biobase, limma, DESeq2, edgeR, clusterProfiler) for a single workflow: download, QC, normalization, batch correction, differential expression, WGCNA, pathway enrichment, PPI, and machine learning. Uses common data structures (ExpressionSet, DGEList) for interoperability. For a full dependency tree (including STRINGdb + PPI helpers), use BiocManager::install("GExPipe", dependencies = TRUE). STRING data are downloaded on first PPI use (internet required); they cannot be bundled in the package. Microarray CEL normalization uses affy and/or oligo when supplementary CEL files are available.

Installation

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("GExPipe")

Details

MaintainerSafa Rafique <safa.sandhu@gmail.com>
AuthorSafa Rafique [aut, cre] (ORCID: <https://orcid.org/0000-0003-2646-8106>), Naeem Mahmood Ashraf [aut], Prof. Dr. Muhammad Farooq Sabar [aut]
LicenseMIT + file LICENSE
URLhttps://github.com/safarafique/GExPipe
Bug Reportshttps://github.com/safarafique/GExPipe/issues
System RequirementsGNU make
Downloads rank54
Source branchdevel
biocViewsDifferentialExpression, GeneExpression, Microarray, Network, NetworkEnrichment, Normalization, Pathways, RNASeq, ShinyApps, Software, Visualization

Documentation

Download

Follow the installation instructions to use this package in your R session.

Source packageGExPipe_0.99.51.tar.gz
Windows binary (x86_64)GExPipe_0.99.51.zip
macOS binary (arm64)GExPipe_0.99.51.tgz
macOS binary (x86_64)GExPipe_0.99.51.tgz
Dependencies

Depends: R (>= 4.5.0)

Imports: affy (>= 1.84.0), AnnotationDbi (>= 1.64.0), Biobase (>= 2.62.0), biomaRt (>= 2.58.0), caret (>= 6.0.94), circlize (>= 0.4.16), cli (>= 3.6.0), clusterProfiler (>= 4.10.0), data.table (>= 1.15.0), DESeq2 (>= 1.42.0), dplyr (>= 1.1.0), DT (>= 0.30), dynamicTreeCut (>= 1.63.1), edgeR (>= 4.0.0), enrichplot (>= 1.22.0), GEOquery (>= 2.70.0), ggplot2 (>= 3.4.0), ggpubr (>= 0.6.0), ggraph (>= 2.2.0), ggrepel (>= 0.9.5), glmnet (>= 4.1.0), glue (>= 1.6.0), gridExtra (>= 2.3), igraph (>= 2.0.0), lifecycle (>= 1.0.0), limma (>= 3.58.0), Matrix (>= 1.6.0), msigdbr (>= 7.5.1), oligo (>= 1.66.0), org.Hs.eg.db (>= 3.17.0), parallel, methods, pheatmap (>= 1.0.12), pillar (>= 1.9.0), pROC (>= 1.18.0), R.utils (>= 2.12.0), randomForest (>= 4.7.1), RColorBrewer (>= 1.1.3), Rcpp (>= 1.0.12), reshape2 (>= 1.4.4), rlang (>= 1.1.0), rms (>= 6.7.0), scales (>= 1.3.0), shiny (>= 1.8.0), shinydashboard (>= 0.7.2), shinyjs (>= 2.1.0), STRINGdb (>= 2.14.0), sva (>= 3.50.0), tibble (>= 3.2.0), tidyr (>= 1.3.0), tidygraph (>= 1.3.0), UpSetR (>= 1.4.0), vctrs (>= 0.6.0), VennDiagram (>= 1.7.0), WGCNA (>= 1.72), withr (>= 2.5.0), xgboost (>= 1.7.0)

Suggests: BiocCheck, BiocManager, BiocStyle, Boruta (>= 8.0.0), bslib, car (>= 3.1.0), chromote, cicerone (>= 1.0.4), corrplot (>= 0.92), crosstalk, dcurves (>= 0.5.0), devtools, fontawesome, htmltools, htmlwidgets, kernlab (>= 0.9.32), knitr, mixOmics (>= 6.26.0), pak, pkgload, rmarkdown, remotes, SHAPforxgboost (>= 0.1.0), shinytest2, stringi, testthat