backgroundCorrectSingleSection {beadarray} | R Documentation |
Function to perform a basic bead-level background correction using a defined set of foreground and background intensities.
backgroundCorrectSingleSection(BLData, array = 1, fg="Grn", bg="GrnB", newName = "Grn.bc")
BLData |
a |
array |
the number of the array-section to be corrected |
fg |
the name under which the foreground intensities are stored |
bg |
the name under which the background intensities are stored |
newName |
Name to store the corrected intensities |
This function takes two attributes of a bead-level object and returns that bead-level object with an additional attribute consisting of the difference of the other two. We anticipate this being used as a simple background correction step, returning the difference between foreground and background intensities to be used as the specific intensity associated with a bead.
Functions to perform more advanced background correction steps can easily be constructed after consideration of this function.
beadLevelData
object with modified beadData slot for the particular section
Mark Dunning
## Not run: if(require(beadarrayExampleData)){ data(exampleBLData) head(exampleBLData[[1]]) for(i in 1:2){ exampleBLData = backgroundCorrectSingleSection(exampleBLData, array=i) } head(exampleBLData[[1]]) } else { stop("You will need the beadarrayExampleData package to run this example") } ## End(Not run)