mapFeaturesToCRMs {LedPred} | R Documentation

## R interface to bed_to_matrix REST in server

### Description

The `mapFeaturesToCRMs` function allows the user to create a training set matrix to build a predictive model. The training set is composed of positive regions (known to be involved in the pathway of interest) and negative regions (randomly picked or known to not be involved in the pathway of interest) that will be described (scored) by features. Three types of features file format are accepted: Position specific scoring matrices modeling motifs recognised by transcription factors, bed files containing region coordinates for any discrete feature (NGS peaks, conservation blocks) and wig/bigWig files containing signal data. This script has been tested with version 0.99 of the online server. Go here to see current version of the server http://ifbprod.aitorgonzalezlab.org/map_features_to_crms.php

### Usage

      mapFeaturesToCRMs(URL = "http://ifbprod.aitorgonzalezlab.org/map_features_to_crms.php",
      positive.bed = NULL, genome = NULL, negative.bed = NULL,
      shuffling = NULL, background.seqs = NULL, genome.info = NULL,
      pssm = NULL, background.freqs = NULL, ngs = NULL, bed.overlap = NULL,
      my.values = NULL, feature.ranking = NULL, feature.nb = NULL,
      crm.feature.file = NULL, stderr.log.file = NULL, stdout.log.file = NULL)

### Arguments

`URL` | URL of the server REST target
`positive.bed` | Positive bed file path. Compulsory
`genome` | Genome code, eg. dm3 for Drosophila Melanogaster. Compulsory
`negative.bed` | Negative bed file path.
`shuffling` | Integer with number of time shuffle background sequences (background.seqs). If negative.bed is NULL and shuffling is set at 0, the feature matrix does not contain negative sequences. It is useful to produce a test set matrix.
`background.seqs` | Background sequences used for shuffling. If shuffling = 0, set this parameter at 0.
`genome.info` | File require for shuffling bed. If shuffling = 0, set this parameter at 0.
`pssm` | Position specific scoring matrices
`background.freqs` | Background frequencies of nucleotides in genome
`ngs` | NGS (bed and wig) files
`bed.overlap` | Minimal overlap as a fraction of query sequence with NGS bed peak. Equivalent with intersectBed -f argument. Default 1bp.
`my.values` | Bed file where fourth column are values to append to the SVM matrix
`feature.ranking` | File with ranked features (Output of rankFeatures). It is used for scoring a query bed file
`feature.nb` | Integer with feature.nb
`crm.feature.file` | Path to feature matrix file
`stderr.log.file` | Path to error log
`stdout.log.file` | Path to standard output log

### Value

A list

`feature.matrix` | a data frame where each row is a region and each column a feature, each cell carry a score, the first column is the response vector
`stdout.log` | Standard output log of mapFeaturesToCRMs script in server
`stderr.log` | Standard error log of mapFeaturesToCRMs script in server

### Examples

    ## Not run:
     dirPath <- system.file("extdata", package="LedPred")
     file.list <-   list.files(dirPath, full.names=TRUE)
     background.freqs <- file.list[grep("freq", file.list)]
     positive.regions <-  file.list[grep("positive", file.list)]
     negative.regions <-  file.list[grep("negative", file.list)]
     TF.matrices <-  file.list[grep("tf", file.list)]
     ngs.path <- system.file("extdata/ngs", package="LedPred")
     ngs.files=list.files(ngs.path, full.names=TRUE)
     crm.features.list <- mapFeaturesToCRMs(positive.bed=positive.regions,
         negative.bed=negative.regions,  background.freqs=background.freqs,
         pssm=TF.matrices, genome="dm3", ngs=ngs.files,
         crm.feature.file = "crm.features.tab",
         stderr.log.file = "stderr.log", stdout.log.file = "stdout.log")
     names(crm.features.list)
     class(crm.features.list$crm.features)
     crm.features.list$stdout.log
     crm.features.list$stderr.log

    ## End(Not run)

[Package LedPred version 1.18.0 Index]