backgroundSignal {ChIPanalyser}R Documentation

Accessor method for the backgroundSignal slot in a occupancyProfileParameters object.

Description

Extract or access the backgroundSignal slot in a occupancyProfileParameters object.

Usage

backgroundSignal(object)

Arguments

object

object is an occupancyProfileParameters

Details

Default Value: 0

When computing computeOccupancy, a ChIP-seq background signal is used to scale Occupancy by considering both a backgroundSignal and a maxSignal. The backgroundSignal is also used to nomalise occupancies against maxOccupancy. The backgroundSignal usually comes from experimental data and is provided by user. As a general rule, if ChIP-seq data is available and will be used in computeChipProfile , profileAccuracyEstimate or plotOccupancyProfile, it is advised to use the backgroundSignal from this data. We strongly encourage to set values when building a occupancyProfileParameters object.

Value

Returns a backgroundSignal of a occupancyProfileParameters object.

Author(s)

Patrick C.N. Martin <pm16057@essex.ac.uk>

References

Zabet NR, Adryan B (2015) Estimating binding properties of transcription factors from genome-wide binding profiles. Nucleic Acids Res., 43, 84–94.

Examples

# Building occupancyProfileParameters object
OPP <- occupancyProfileParameters()
#Viewing single value in object
backgroundSignal(OPP)



[Package ChIPanalyser version 1.6.0 Index]