ctlSeparatn {phenoDist} | R Documentation |
This function calculates the Z'-factor between negative and positive controls, based on phenotypic distance measurements.
ctlSeparatn(x, pheno, neg='rluc', pos='ubc', ...)
x |
An |
pheno |
A numeric vector for distances to negative controls, as returned from |
neg |
A character string to identify negative controls. |
pos |
A character string to identify positive controls. |
... |
Additional arguments to be passed to the |
Phenotypes of negative and positive controls are defined as their phenotypic distance to negative controls. The Z'-factor is a metric measuring the separation between negative controls and positive controls (Zhang et al. 1999). This function calls the Zprime
function of the imageHTS
package to calculate the Z'-factor. Please see Zprime
for detailed description of the definition and calculation of the Z'-factor.
The Z'-factor value.
Xian Zhang
J. H. Zhang, T. D. Chung, K. R. Oldenburg. A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J Biomol Screening, 1999.
distToNeg
, Zprime
## see distToNeg.