EHtrans-class {flowCore} | R Documentation |
EH transformation of a parameter is defined by the function
EH(parameter,a,b)= 10^{(\frac{parameter}{a})} + \frac{b*parameter}{a}-1 ~~~~parameter>=0
-10^{(\frac{-parameter}{a})} + \frac{b*parameter}{a}+1 ~~~~parameter<0
Objects can be created by calls to the constructor
EHtrans(parameters,a,b,transformationId)
.Data
:Object of class "function"
~~
a
:Object of class "numeric"
-numeric constant greater than zero
b
:Object of class "numeric"
-numeric constant greater than zero
parameters
:Object of class "transformation"
- flow parameter to be
transformed
transformationId
:Object of class "character"
- unique ID to reference the transformation
Class "singleParameterTransform"
, directly.
Class "transform"
, by class "singleParameterTransform", distance 2.
Class "transformation"
, by class "singleParameterTransform", distance 3.
Class "characterOrTransformation"
, by class "singleParameterTransform", distance 4.
No methods defined with class "EHtrans" in the signature.
The transformation object can be evaluated using the eval method by passing the data frame as an argument.The transformed parameters are returned as a matrix with a single column. (See example below)
Gopalakrishnan N, F.Hahne
Gating-ML Candidate Recommendation for Gating Description in Flow Cytometry V 1.5
hyperlog
dat <- read.FCS(system.file("extdata","0877408774.B08", package="flowCore")) eh1<-EHtrans("FSC-H",a=1250,b=4,transformationId="eh1") transOut<-eval(eh1)(exprs(dat))