negativeFactor {NanoStringDiff} | R Documentation |
Negative size factors can be used to adjust background niose for each sample.
## S4 method for signature 'NanoStringSet' negativeFactor(object) ## S4 replacement method for signature 'NanoStringSet,numeric' negativeFactor(object) <- value
object |
A NanoStringSet object. |
value |
A vector of background noise. |
Accurate estimation of system background is essential for DE detection analysis. Each code set in the nCounter Analyzer includes several negatives control genes for which no tranCounterript is expected to be present. We use these spike-in negative control genes to estimate background noise for each sample
A vector contain background noise
Hong Wang <hong.wang@uky.edu> chi wang <chi.wang@uky.edu>
negativeControl
data(NanoStringData) ## obtain negative factors negativeFactor(NanoStringData) ## assign a vector n=ncol(exprs(NanoStringData)) lamda=rpois(n,10) negativeFactor(NanoStringData)=lamda