extractBFArea {CONFESS} | R Documentation |
It estimates the spot or capture site location by BF image modelling.
extractBFArea(cc, img, area, BFarea)
cc |
Data matrix. An estimated spot center. |
img |
Data matrix. The matrix of image data from a channel. |
area |
Data matrix. The bright (spot) coordinates around the spot center. |
BFarea |
Integer. Defines a rectangular pseudo-spot size whose fluorescence will be estimated. |
The area identified by BF image modelling