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esATAC

An Easy-to-use Systematic pipeline for ATACseq data analysis

Bioconductor version: 3.23 · Package version: 1.34.0

This package provides a framework and complete preset pipeline for quantification and analysis of ATAC-seq Reads. It covers raw sequencing reads preprocessing (FASTQ files), reads alignment (Rbowtie2), aligned reads file operations (SAM, BAM, and BED files), peak calling (F-seq), genome annotations (Motif, GO, SNP analysis) and quality control report. The package is managed by dataflow graph. It is easy for user to pass variables seamlessly between processes and understand the workflow. Users can process FASTQ files through end-to-end preset pipeline which produces a pretty HTML report for quality control and preliminary statistical results, or customize workflow starting from any intermediate stages with esATAC functions easily and flexibly.

Installation

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("esATAC")

Details

MaintainerZheng Wei <wzweizheng@qq.com>
AuthorZheng Wei, Wei Zhang
LicenseGPL-3 | file LICENSE
URLhttps://github.com/wzthu/esATAC
Bug Reportshttps://github.com/wzthu/esATAC/issues
System RequirementsC++11
Downloads rank654
Source branchRELEASE_3_23
biocViewsATACSeq, Alignment, Coverage, DNASeq, DNaseSeq, ImmunoOncology, Preprocessing, QualityControl, Sequencing, Software

Documentation

Download

Follow the installation instructions to use this package in your R session.

Source packageesATAC_1.34.0.tar.gz
Windows binary (x86_64)esATAC_1.34.0.zip
macOS binary (arm64)esATAC_1.34.0.tgz
macOS binary (x86_64)esATAC_1.34.0.tgz
Dependencies

Depends: R (>= 4.0.0), Rsamtools, GenomicRanges, ShortRead, pipeFrame

Imports: Rcpp (>= 0.12.11), methods, knitr, Rbowtie2, rtracklayer, ggplot2, Biostrings, ChIPseeker, clusterProfiler, igraph, rJava, magrittr, digest, BSgenome, AnnotationDbi, GenomicAlignments, GenomicFeatures, R.utils, Seqinfo, BiocGenerics, S4Vectors, IRanges, rmarkdown, tools, VennDiagram, grid, JASPAR2018, TFBSTools, grDevices, graphics, stats, utils, parallel, corrplot, BiocManager, motifmatchr

LinkingTo: Rcpp

Suggests: BSgenome.Hsapiens.UCSC.hg19, TxDb.Hsapiens.UCSC.hg19.knownGene, org.Hs.eg.db, testthat, webshot, prettydoc