DAPAR user manual

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Introduction

The DAPAR and Prostar packages are a series of software dedicated to the processing of proteomics data. More precisely, they are devoted to the analysis of quantitative datasets produced by bottom-up discovery proteomics experiments with a LC-MS/MS pipeline (Liquid Chromatography and Tandem Mass spectrometry). DAPAR (Differential Analysis of Protein Abundance with R) is an R package that contains all the necessary functions to process the data in command lines. It can be used on its own; or as a complement to the numerous Bioconductor packages () it is compliant with; or through the Prostar interface. Prostar (Proteomics statistical analysis with R) is a web interface based on Shiny technology () that provides GUI (Graphical User Interfaces) to all the DAPAR functionalities, so as to guide any practitioner that is not comfortable with R programming through the complete quantitative analysis process. The experiment package DAPARdata contains many datasets that can be used as examples.

sessionInfo()
## R version 4.5.3 (2026-03-11)
## Platform: x86_64-pc-linux-gnu
## Running under: Ubuntu 24.04.4 LTS
## 
## Matrix products: default
## BLAS:   /usr/lib/x86_64-linux-gnu/openblas-pthread/libblas.so.3 
## LAPACK: /usr/lib/x86_64-linux-gnu/openblas-pthread/libopenblasp-r0.3.26.so;  LAPACK version 3.12.0
## 
## locale:
##  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
##  [3] LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8    
##  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
##  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
##  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       
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## time zone: Etc/UTC
## tzcode source: system (glibc)
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## attached base packages:
## [1] stats     graphics  grDevices utils     datasets  methods   base     
## 
## other attached packages:
## [1] BiocStyle_2.39.0
## 
## loaded via a namespace (and not attached):
##  [1] digest_0.6.39       R6_2.6.1            fastmap_1.2.0      
##  [4] xfun_0.57           maketools_1.3.2     cachem_1.1.0       
##  [7] knitr_1.51          htmltools_0.5.9     rmarkdown_2.31     
## [10] buildtools_1.0.0    lifecycle_1.0.5     cli_3.6.6          
## [13] sass_0.4.10         jquerylib_0.1.4     compiler_4.5.3     
## [16] sys_3.4.3           tools_4.5.3         bslib_0.10.0       
## [19] evaluate_1.0.5      yaml_2.3.12         otel_0.2.0         
## [22] BiocManager_1.30.27 jsonlite_2.0.0      rlang_1.2.0