reducedFlowframe,MarginEvents-method {cyanoFilter} | R Documentation |
accesor method for reduced flowframe (MarginEvent class)
## S4 method for signature 'MarginEvents' reducedFlowframe(x)
x |
an object of class MarginEvents |
reduced Flowframe method for MarginEvents
flowfile_path <- system.file("extdata", "B4_18_1.fcs", package = "cyanoFilter", mustWork = TRUE) flowfile <- flowCore::read.FCS(flowfile_path, alter.names = TRUE, transformation = FALSE, emptyValue = FALSE, dataset = 1) flowfile_nona <- cyanoFilter::noNA(x = flowfile) flowfile_noneg <- cyanoFilter::noNeg(x = flowfile_nona) flowfile_logtrans <- cyanoFilter::lnTrans(x = flowfile_noneg, c('SSC.W', 'TIME')) cells_nonmargin <- cellMargin(flowframe = flowfile_logtrans, Channel = 'SSC.W', type = 'estimate', y_toplot = "FSC.HLin") reducedFlowframe(cells_nonmargin)