.singlePrepUMI4C {UMI4Cats} | R Documentation |
Prepar fastq files at a given barcode.
.singlePrepUMI4C( fq_R1, fq_R2, bait_seq, bait_pad, res_enz, prep_dir, numb_reads = 1e+09 )
fq_R1 |
Fastq file R1. |
fq_R2 |
Fastq file R2. |
bait_seq |
Character containing the bait primer sequence. |
bait_pad |
Character containing the pad sequence (sequence between the bait primer and the restriction enzyme sequence). |
res_enz |
Character containing the restriction enzyme sequence. |
prep_dir |
Prep directory. |
numb_reads |
Number of lines from the FastQ file to load in each loop. If having memory size problems, change it to a smaller number. Default=1e9. |
Creates a compressed FASTQ file in wk_dir/prep
named
basename(Fastq)).fq.gz
, containing the filtered reads with the UMI
sequence in the header. A data.frame object with the statisitics is also
returned.